Background: Many cancer therapies currently available are teratogenic in nature. Several patients cared for within our community ambulatory oncology infusion center are women of childbearing potential (WOCP). Pregnancy testing of these women within our oncology infusion center was sporadic at best. There currently are no standardized guidelines related to pregnancy testing of these women. Methods: A retrospective chart analysis of WOCP receiving chemotherapy was done to determine baseline pregnancy screening rates. Subsequently a protocol was developed in which WOCP was defined as women under 50 years of age, without a history of hysterectomy or tubal ligation. The nurse-driven protocol allowed the infusion nursing staff to order a pregnancy screen to be done within the infusion center if testing had not already taken place within 48 hours of initiation of treatment. Following protocol implementation a retrospective chart analysis was performed to re-examine screening rates. Results: Utilizing patients with an active treatment plan within a one month time period, retrospective analysis of pregnancy screening prior to initiation of chemotherapy was performed. Baseline pregnancy screening rate was 21%. Point of care pregnancy equipment was obtained and nursing staff was educated on the protocol to include a medical delegation. Post-implementation chart analysis of WOCP initiating chemotherapy within a six-week timeframe resulted in improved pregnancy screening to 57% compliance. Continued education and clarification of the protocol was provided to the nurses in an effort to further improve compliance. Conclusions: Lack of standard guidelines regarding pregnancy screening of WOCP led to the development and implementation of a protocol for pregnancy screening for this at-risk population. Medical delegation allowed nursing to initiate the screening for these patients allowing for improvements in the safety and quality of patient care provided.