Background: Cervical cancer (CC) is the fourth cause of malignancy-related deaths among women globally. In Saudi Arabia, it is the 8th most prevalent cancer and the 7^th cause of cancer mortality among women between the ages of 15–44. There is a limited national data on the awareness of CC, human papilloma virus (HPV), and knowledge of screening and prevention. We present the largest national study to date investigating knowledge of Saudi women toward CC, the HPV vaccine, Pap smear, and influencing factors of their application. Methods: A structured questionnaire of 22 questions included demographic data, CC awareness and associated risk factors, evaluation of Pap-smear and HPV vaccine knowledge and their application was constructed and validated. Participants were exclusively female. It was distributed across the country and scored according to a pre-defined scoring system. Influencing factors of CC, Pap-smear and HPV vaccine knowledge and awareness were examined. A regression model to predict the possibility of performing Pap-smear was generated. P-value of 0.05 and less indicated statistical significance in all tests. Results: 2539 female participants completed the questionnaire with (41%) aged 18 - 25 years old. A sizable portion were from the central region (30.4%) and a majority had a university degree (73.1%). 65% of the participants were sexually active. Two thirds of the participants have heard about Pap-smear and its role in detecting CC but only 20% took the test. Although one third knew that the HPV vaccine can prevent CC, only 2% received the vaccination. Poor knowledge on locating the Pap-smear and HPV vaccine were reported among participants. 12% reported that health campaigns were their source of information. Factors associated with adequate knowledge are listed in table. Age predicted the possibility of performing Pap-smear with participants of 18 - 25 years old are more likely to take the test (P=0.00). Conclusions: This large study evaluated the knowledge and awareness of CC, Pap-smear, and the HPV vaccine across the country. It demonstrated the need for better advocacy and for a nation-wide campaigning to further utilize early detection and preventive procedures.